**Figure 2.** Fig 1. Sphere-forming efficiency decreases following radiation. A single 5 Gy dose of radiation (IR d30) was given to 4–6 week old female FVB mice and parotid glands were collected 30 days following radiation for sphere formation assay. Representative bright field images of salispheres grown from untreated control (UT) and irradiated (IR d30) glands in serum-free media at different time points in culture (A). Number of salispheres was quantified per 6 wells from 3 primary cell preparations per treatment group at days 4, 5, 6, 7, and 14 in culture and expressed as average ± SEM (B). Representative graphs showing the distribution of salisphere sizes among all the salispheres counted from one UT and IR d30 primary sphere preparation (C). P values were obtained with 2-sided unpaired t-test (n 3), p < .01, p < .001, p < .0001. Scale bar 50μm.

विवरण

Sphere-forming efficiency of parotid-derived cells decreases significantly following a single 5 Gy radiation dose, quantified at day 30 post-irradiation.

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Figure 3

Proliferation rates of salisphere cultures from untreated and irradiated parotid glands are compared under serum-free conditions, showing similar growth kinetics despite radiation damage.

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Figure 4

Fetal bovine serum supplementation increases the sphere-forming efficiency of irradiated parotid-derived cells, as shown by representative bright-field microscopy images and quantification.

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Figure 5

Proliferation assays confirm comparable growth rates between untreated and irradiated salisphere cultures supplemented with FBS, indicating that reduced sphere formation is not due to proliferation deficits.

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Figure 6

Expression of salivary stem and progenitor cell markers in salisphere cultures demonstrates that both untreated and irradiated cells maintain their progenitor phenotype.

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Figure 7

Post-radiation IGF-1 treatment significantly enhances sphere-forming efficiency of irradiated parotid cells, suggesting growth factor administration as a strategy to restore salivary function.

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Figure 2

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Source Paper

Administration of growth factors promotes salisphere formation from irradiated parotid salivary glands.

PloS one (2018)

PMID: 29590144

DOI: 10.1371/journal.pone.0193942

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![Figure 2: Sphere-forming efficiency of parotid-derived cells decreases significantly following a single 5 Gy radiation dose, quantified at day 30 post-irradiation.](https://pdfs.citedhealth.com/figures/29590144/162.png)

> Source: Vicky T Nguyen et al. "Administration of growth factors promotes salisphere formation from irradiated p." *PloS one*, 2018. PMID: [29590144](https://pubmed.ncbi.nlm.nih.gov/29590144/)

<figure>
  <img src="https://pdfs.citedhealth.com/figures/29590144/162.png" alt="Sphere-forming efficiency of parotid-derived cells decreases significantly following a single 5 Gy radiation dose, quantified at day 30 post-irradiation." />
  <figcaption>Figure 2. Sphere-forming efficiency of parotid-derived cells decreases significantly following a single 5 Gy radiation dose, quantified at day 30 post-irradiation.<br>  Source: Vicky T Nguyen et al. "Administration of growth factors promotes salisphere formation from irradiated p." <em>PloS one</em>, 2018. PMID: <a href="https://pubmed.ncbi.nlm.nih.gov/29590144/">29590144</a></figcaption>
</figure>

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