**Figure 6.** Fig 5. Expression of salivary stem/progenitor markers by salisphere cells. Untreated and irradiated parotid-derived salispheres, maintained under different FBS concentrations, were fixed after 7 days in culture and stained for Keratin 5 (K5) or Keratin 14 (K14) (green). Representative confocal immunofluorescence images are shown (A-B). Representative immunoblot of protein extracts from untreated and irradiated salispheres grown under different FBS concentration medium, collected at day 7 in culture, and probed for Keratin 5 and Keratin 14 (C-D). On day 7 of culture, salispheres were labelled with EdU for 1 hour in vitro, fixed, and dual stained for EdU and Keratin 5 or Keratin 14. Percentage of dual EdU-K14+ or Edu-K5+ cells was quantified from 20 salispheres, maintained under different FBS concentrations, for both treatment groups and expressed as mean percent of K14+ or K5+ proliferating cells (± SEM) within an individual salisphere. Significant differences (p< 0.05) were determined using a one-way ANOVA followed by post-hoc Tukey multiple-comparison tests. Groups with the same letter are not significant different from each other (E-F). Scale bar = 50μm.

描述

Expression of salivary stem and progenitor cell markers in salisphere cultures demonstrates that both untreated and irradiated cells maintain their progenitor phenotype.

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Figure 2

Sphere-forming efficiency of parotid-derived cells decreases significantly following a single 5 Gy radiation dose, quantified at day 30 post-irradiation.

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Figure 3

Proliferation rates of salisphere cultures from untreated and irradiated parotid glands are compared under serum-free conditions, showing similar growth kinetics despite radiation damage.

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Figure 4

Fetal bovine serum supplementation increases the sphere-forming efficiency of irradiated parotid-derived cells, as shown by representative bright-field microscopy images and quantification.

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Figure 5

Proliferation assays confirm comparable growth rates between untreated and irradiated salisphere cultures supplemented with FBS, indicating that reduced sphere formation is not due to proliferation deficits.

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Figure 7

Post-radiation IGF-1 treatment significantly enhances sphere-forming efficiency of irradiated parotid cells, suggesting growth factor administration as a strategy to restore salivary function.

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Figure 6

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Source Paper

Administration of growth factors promotes salisphere formation from irradiated parotid salivary glands.

PloS one (2018)

PMID: 29590144

DOI: 10.1371/journal.pone.0193942

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![Figure 6: Expression of salivary stem and progenitor cell markers in salisphere cultures demonstrates that both untreated and irradiated cells maintain their progenitor phenotype.](https://pdfs.citedhealth.com/figures/29590144/204.png)

> Source: Vicky T Nguyen et al. "Administration of growth factors promotes salisphere formation from irradiated p." *PloS one*, 2018. PMID: [29590144](https://pubmed.ncbi.nlm.nih.gov/29590144/)

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  <img src="https://pdfs.citedhealth.com/figures/29590144/204.png" alt="Expression of salivary stem and progenitor cell markers in salisphere cultures demonstrates that both untreated and irradiated cells maintain their progenitor phenotype." />
  <figcaption>Figure 6. Expression of salivary stem and progenitor cell markers in salisphere cultures demonstrates that both untreated and irradiated cells maintain their progenitor phenotype.<br>  Source: Vicky T Nguyen et al. "Administration of growth factors promotes salisphere formation from irradiated p." <em>PloS one</em>, 2018. PMID: <a href="https://pubmed.ncbi.nlm.nih.gov/29590144/">29590144</a></figcaption>
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