Sedative and hypnotic effects of compound Anshen essential oil inhalation for insomnia.
Study Design
- 研究タイプ
- In Vitro
- 介入
- Sedative and hypnotic effects of compound Anshen essential oil inhalation for insomnia. Compound Anshen essential oil (inhaled); dose not specified
- 比較対照
- Placebo
- 効果の方向
- Positive
- バイアスリスク
- Unclear
Abstract
BACKGROUNDS: The chemical composition of many essential oils indicates that they have sedative and hypnotic effects, but there is still a lack of systematic studies on the sedative and hypnotic effects of essential oils. In addition, aromatherapy does not seem to have the side effects of many traditional psychotropic substances, which is clearly worthwhile for further clinical and scientific research. The clinical application of essential oils in aromatherapy has received increasing attention, and detailed studies on the pharmacological activities of inhaled essential oils are increasingly needed. HYPOTHESIS/PURPOSE: As insomniacs are usually accompanied by symptoms of depression and anxiety of varying degrees, based on the theory of aromatherapy of Traditional Chinese Medicine, this experiment is to study a Compound Anshen essential oil that is compatible with Lavender essential oil, Sweet Orange essential oil, Sandalwood essential oil and other aromatic medicine essential oils with sedative and hypnotic effects, anti-anxiety and anti-depression effects. To study the sedative and hypnotic effects of Compound Anshen essential oil inhaled and the main chemical components of Compound Anshen essential oil, and to compare and analyze the pharmacodynamics of diazepam, a commonly used drug for insomnia. METHODS: The Open field test and Pentobarbital-induced sleep latency and sleep time experiments were used to analyze and compare the sedative and hypnotic effects of inhaling Compound Anshen essential oil and the administration of diazepam on mice. The changes of 5-HT and GABA in mouse brain were analyzed by Elisa. The main volatile constituents of Compound Anshen essential oil were analyzed by gas chromatography-mass spectrometry (GC-MS). RESULTS: Inhalation of Compound Anshen essential oil can significantly reduce the spontaneous activity of mice, reduce latency of sleeping time and prolong duration of sleeping time. The results of enzyme-linked immunosorbent assay showed that Compound Anshen essential oil can increase the content of 5-HT and GABA in mouse brain. The main volatile chemical constituents of the Compound Anshen essential oil are D-limonene (24.07%), Linalool (21.98%), Linalyl acetate (15.37%), α-Pinene (5.39%), and α-Santalol (4.8%). CONCLUSION: The study found that the inhalation of Compound Anshen essential oil has sedative and hypnotic effect. This study provides a theoretical basis for further research and development of the sedative and hypnotic effects of Compound Anshen essential oil based on the theory of aromatherapy.
Full Text
Figures
Fig. 1
Study design for evaluating the sedative and hypnotic effects of compound Anshen essential oil inhalation in insomnia subjects is outlined.
flowchart
Fig. 2
Sleep quality measurements comparing Anshen essential oil aromatherapy with control conditions are presented, based on validated insomnia assessment scales.
chart
Fig. 3
EEG or polysomnographic sleep architecture data from the essential oil intervention study are displayed, tracking changes in sleep stages.
chart
Fig. 4
Dose-response or temporal effects of Anshen essential oil inhalation on sleep parameters are charted across the study period.
chart
Fig. 5
Summary outcomes from the compound Anshen essential oil trial are presented, integrating subjective sleep quality with objective physiological measures.
chartTables
Table 1
| No. | Retention Time (min) | Compound | Chemical formula | molecular weight | Relative Amount (%) |
|---|---|---|---|---|---|
| 1 | 5.943 | α-Thujene | C10H16 | 136 | 0.63 |
| 2 | 6.064 | α-Pinene | C10H16 | 136 | 5.39 |
| 3 | 6.482 | Sabinene | C10H16 | 136 | 0.28 |
| 4 | 6.733 | β-Phellandrene | C10H16 | 136 | 0.58 |
| 5 | 6.796 | β-Pinene | C10H16 | 136 | 0.4 |
| 6 | 6.927 | 3-Octanone | C8H16O | 128 | 0.66 |
| 7 | 6.998 | β-Myrcene | C10H16 | 136 | 1.04 |
| 8 | 7.079 | Sarcosine | C3H7NO2 | 89 | 0.25 |
| 9 | 7.358 | Hexyl acetate | C4H8O2 | 144 | 0.51 |
| 10 | 7.585 | Cymene | C10H14 | 134 | 0.79 |
| 11 | 7.657 | D-Limonene | C10H16 | 136 | 24.07 |
| 12 | 7.771 | trans-β-Ocimene | C10H16 | 136 | 1.81 |
| 13 | 7.948 | β-Ocimene | C10H16 | 136 | 1.17 |
| 14 | 8.148 | γ-Terpinene | C10H16 | 136 | 0.41 |
| 15 | 8.803 | Linalool | C10H18O | 154 | 21.98 |
| 16 | 10.076 | 4-Terpineol | C10H18O | 154 | 1.73 |
| 17 | 8.953 | 1-Octen-3-yl acetate | C10H18O2 | 170 | 0.63 |
| 18 | 10.076 | Terpinen-4-ol | C10H18O | 154 | 1.73 |
| 19 | 10.206 | Cyclobutanol | C4H8O | 72 | 0.26 |
| 20 | 10.28 | α-Terpineol | C10H18O | 154 | 0.95 |
| 21 | 10.812 | Citronellol | C10H20O | 156 | 1.42 |
| 22 | 11.171 | Linalyl acetate | C12H20O2 | 196 | 15.37 |
| 23 | 11.666 | lavandulyl acetate | C12H20O2 | 196 | 1.8 |
| 24 | 12.971 | Geranyl acetate | C12H20O2 | 196 | 0.51 |
| 25 | 13.624 | Caryophyllene | C15H24 | 204 | 1.71 |
| 26 | 13.958 | β-Famesene | C15H24 | 204 | 1.57 |
| 27 | 14.094 | β-santalene | C15H24 | 204 | 0.38 |
| 28 | 16.839 | α-Santalol | C15H24O | 220 | 4.8 |
| 28 | 19.023 | Nonadecane | C19H40 | 268 | 1.21 |
| 29 | 19.702 | Dibutyl phthalate | C16H22O4 | 278 | 1.21 |
| 30 | 20.636 | Eicosane | C20H42 | 282 | 0.4 |
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