Carbohydrate fermentation by Clostridium difficile.

S Nakamura, S Nakashio, K Yamakawa, N Tanabe, S Nishida

Other Microbiology and immunology 1982 41 citazioni

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Study Design

Tipo di studio: Other
Popolazione: general population
Durata: 0.3 weeks

Intervento: Carbohydrate fermentation by Clostridium difficile. None
Comparatore: None
Esito primario: growth
Direzione dell'effetto: Mixed
Rischio di bias: Moderate

Abstract

Biochemical properties of Clostridium difficile were reinvestigated for the practical identification of the organism in clinical laboratories. Bacterial growth in 2% proteose peptone medium supplemented with 0.01% L-cysteine.HCl and 0.1% agar supported sufficient growth to read the fermentation results just as well as did pre-reduced anaerobically sterilized medium. Incubation for 2 days was long enough for determining the ability to ferment fructose, glucose, mannitol, mannose, melezitose, and sorbitol. All of the 82 strains liquefied 2% but not 10% gelatin. The significance of mannitol fermentation and gelatin liquefaction is stressed since C. difficile is the only species fermenting mannitol among the gelatin-liquefying species of clostridia having subterminal spores.

TL;DR

Bacterial growth in 2% proteose peptone medium supplemented with 0.01% l‐cysteine · HCl and 0.1% agar supported sufficient growth to read the fermentation results just as well as did pre‐reduced anaerobically sterilized medium.