**Figure 5.** Fig 4. Similar proliferation rates are observed in untreated and irradiated salisphere cultures supplemented with FBS. Untreated and irradiated parotid-derived salispheres, maintained under different FBS concentrations, were fixed after 4 and 7 days in culture and stained for Ki-67 (green). Representative confocal immunofluorescence images are shown (A-B). Percentage of Ki67+ proliferating cells was quantified from 20 salispheres, of different sizes (<50μm, 50–150μm, >150μm) and maintained under different FBS concentration (2.5% and 10%), at days 4 and 7 for both treatment groups and expressed as average ± SEM. At day 4 in 10% FBS culture condition, large-sized salispheres (>150μm) were rarely detected. Likewise, small-sized salispheres (<50μm) were rarely observed at day 7 in culture. Thus, these analyses were not determined (n.d.). Significant differences (p< 0.05) were determined using a one-way ANOVA followed by post-hoc Tukey multiple-comparison tests on the log transformed data of Fig 4C and 4D. Groups with the same letter are not significant different from each other. (C-D). Scale bar 50μm.

Açıklama

Proliferation assays confirm comparable growth rates between untreated and irradiated salisphere cultures supplemented with FBS, indicating that reduced sphere formation is not due to proliferation deficits.

More Figures from This Paper

Figure 2

Sphere-forming efficiency of parotid-derived cells decreases significantly following a single 5 Gy radiation dose, quantified at day 30 post-irradiation.

chart

Figure 3

Proliferation rates of salisphere cultures from untreated and irradiated parotid glands are compared under serum-free conditions, showing similar growth kinetics despite radiation damage.

chart

Figure 4

Fetal bovine serum supplementation increases the sphere-forming efficiency of irradiated parotid-derived cells, as shown by representative bright-field microscopy images and quantification.

photograph

Figure 6

Expression of salivary stem and progenitor cell markers in salisphere cultures demonstrates that both untreated and irradiated cells maintain their progenitor phenotype.

chart

Figure 7

Post-radiation IGF-1 treatment significantly enhances sphere-forming efficiency of irradiated parotid cells, suggesting growth factor administration as a strategy to restore salivary function.

chart

Figure 5

Chart

827 × 629px · 159,5 KB

Source Paper

Administration of growth factors promotes salisphere formation from irradiated parotid salivary glands.

PloS one (2018)

PMID: 29590144

DOI: 10.1371/journal.pone.0193942

Cite This Figure

![Figure 5: Proliferation assays confirm comparable growth rates between untreated and irradiated salisphere cultures supplemented with FBS, indicating that reduced sphere formation is not due to proliferation deficits.](https://pdfs.citedhealth.com/figures/29590144/193.png)

> Source: Vicky T Nguyen et al. "Administration of growth factors promotes salisphere formation from irradiated p." *PloS one*, 2018. PMID: [29590144](https://pubmed.ncbi.nlm.nih.gov/29590144/)

<figure>
  <img src="https://pdfs.citedhealth.com/figures/29590144/193.png" alt="Proliferation assays confirm comparable growth rates between untreated and irradiated salisphere cultures supplemented with FBS, indicating that reduced sphere formation is not due to proliferation deficits." />
  <figcaption>Figure 5. Proliferation assays confirm comparable growth rates between untreated and irradiated salisphere cultures supplemented with FBS, indicating that reduced sphere formation is not due to proliferation deficits.<br>  Source: Vicky T Nguyen et al. "Administration of growth factors promotes salisphere formation from irradiated p." <em>PloS one</em>, 2018. PMID: <a href="https://pubmed.ncbi.nlm.nih.gov/29590144/">29590144</a></figcaption>
</figure>

İlgili Kanıtlar

Magnesium Glycinate için Huzursuz Bacak Sendromu

Derece F · 54 çalışma