Clonal growth of epithelial cells from normal adult human bronchus.

J F Lechner, A Haugen, H Autrup, I A McClendon, B F Trump et al.

Other Cancer research 1981 204 citazioni

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Study Design

Tipo di studio: In Vitro
Popolazione: animal model

Intervento: Clonal growth of epithelial cells from normal adult human bronchus. None
Comparatore: None
Esito primario: growth
Direzione dell'effetto: Mixed
Rischio di bias: Unclear

Abstract

Normal primary epithelial cell cultures devoid of fibroblastic cells have been developed from tissue explants of adult human bronchi. Conditions for clonal growth of secondary cultures of bronchial epithelial cells were optimized by coculturing the human cells with mitomycin C growth-arrested Swiss 3T3 mouse feeder cells, lowering the calcium concentration of medium M199, and supplementing it with hydrocortisone, insulin, cholera toxin, epidermal growth factor, and 1.25% fetal bovine serum. The epithelial cells grew for an average of 35 population doublings and had the normal human karyotype, expressed keratin and blood group antigen epithelial cell markers, metabolized benzo(a)pyrene, and were capable of differentiating into both ciliated and squamous cells. This culture system makes it potentially possible to investigate various aspects of differentiation and carcinogenesis in human bronchial epithelial cells.

TL;DR

Normal primary epithelial cell cultures devoid of fibroblastic cells have been developed from tissue explants of adult human bronchi, making it potentially possible to investigate various aspects of differentiation and carcinogenesis in human bronchial epithelial cells.

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Clonal growth of epithelial cells from normal adult human bronchus.

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Study Design

Abstract

TL;DR

Used In Evidence Reviews

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