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Fig 6. Post-radiation treatment with IGF1 increases sphere-forming efficiency of irradiated parotid-derived cells. A single 5 Gy dose of radiation was given to 4–6 week old or 8 week female FVB mice followed by injections of insulin growth factor 1 (IGF1)
Figure 7. Fig 6. Post-radiation treatment with IGF1 increases sphere-forming efficiency of irradiated parotid-derived cells. A single 5 Gy dose of radiation was given to 4–6 week old or 8 week female FVB mice followed by injections of insulin growth factor 1 (IGF1) on days 31–33 as depicted (A). Stimulated salivary flow rates were determined as described in the materials and methods section on day 60. The graph represents the mean saliva flow rate from 6 mice (8 week old) per treatment group: untreated control (UT), irradiated (IR d60), and IGF1 treated (IR-IGF1). Significant differences ( p<0.05) were determined using a one-way ANOVA followed by a post-hoc Tukey multiple comparison test (B). Thirty days following IGF1 treatment, parotid glands were collected for sphere formation assay. Representative graph of the average number (± SEM) of salispheres from 10 wells per treatment group on day 7 in culture from one IR d60 and IR+IGF primary sphere preparation (C). Representative bright field images of salispheres grown from irradiated (IR d60) and IGF1 treated (IR-IGF1) glands in serum-free media at different time points in culture (D). Irradiated (IR d60) and IGF1 (IR-IGF1) treated parotidderived salispheres were fixed after 7 days in culture and stained for Ki-67 (green). Representative confocal immunofluorescence images are shown (E). Scale bar = 50μm.

Descrizione

Post-radiation IGF-1 treatment significantly enhances sphere-forming efficiency of irradiated parotid cells, suggesting growth factor administration as a strategy to restore salivary function.

More Figures from This Paper

Sphere-forming efficiency of parotid-derived cells decreases significantly following a single 5 Gy radiation dose, quantified at day 30 post-irradiation.

Figure 2

Sphere-forming efficiency of parotid-derived cells decreases significantly following a single 5 Gy radiation dose, quantified at day 30 post-irradiation.

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Proliferation rates of salisphere cultures from untreated and irradiated parotid glands are compared under serum-free conditions, showing similar growth kinetics despite radiation damage.

Figure 3

Proliferation rates of salisphere cultures from untreated and irradiated parotid glands are compared under serum-free conditions, showing similar growth kinetics despite radiation damage.

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Fetal bovine serum supplementation increases the sphere-forming efficiency of irradiated parotid-derived cells, as shown by representative bright-field microscopy images and quantification.

Figure 4

Fetal bovine serum supplementation increases the sphere-forming efficiency of irradiated parotid-derived cells, as shown by representative bright-field microscopy images and quantification.

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Proliferation assays confirm comparable growth rates between untreated and irradiated salisphere cultures supplemented with FBS, indicating that reduced sphere formation is not due to proliferation deficits.

Figure 5

Proliferation assays confirm comparable growth rates between untreated and irradiated salisphere cultures supplemented with FBS, indicating that reduced sphere formation is not due to proliferation deficits.

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Expression of salivary stem and progenitor cell markers in salisphere cultures demonstrates that both untreated and irradiated cells maintain their progenitor phenotype.

Figure 6

Expression of salivary stem and progenitor cell markers in salisphere cultures demonstrates that both untreated and irradiated cells maintain their progenitor phenotype.

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Figure 7

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Source Paper

Administration of growth factors promotes salisphere formation from irradiated parotid salivary glands.

PloS one (2018)

PMID: 29590144

DOI: 10.1371/journal.pone.0193942

Cite This Figure

![Figure 7: Post-radiation IGF-1 treatment significantly enhances sphere-forming efficiency of irradiated parotid cells, suggesting growth factor administration as a strategy to restore salivary function.](https://pdfs.citedhealth.com/figures/29590144/225.png)

> Source: Vicky T Nguyen et al. "Administration of growth factors promotes salisphere formation from irradiated p." *PloS one*, 2018. PMID: [29590144](https://pubmed.ncbi.nlm.nih.gov/29590144/)
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  <img src="https://pdfs.citedhealth.com/figures/29590144/225.png" alt="Post-radiation IGF-1 treatment significantly enhances sphere-forming efficiency of irradiated parotid cells, suggesting growth factor administration as a strategy to restore salivary function." />
  <figcaption>Figure 7. Post-radiation IGF-1 treatment significantly enhances sphere-forming efficiency of irradiated parotid cells, suggesting growth factor administration as a strategy to restore salivary function.<br>  Source: Vicky T Nguyen et al. "Administration of growth factors promotes salisphere formation from irradiated p." <em>PloS one</em>, 2018. PMID: <a href="https://pubmed.ncbi.nlm.nih.gov/29590144/">29590144</a></figcaption>
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