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Fig 3. FBS increases sphere-forming efficiency of irradiated parotid-derived cells. Representative bright field images of salispheres grown from UT (untreated) and IR d30 (irradiated) glands in serum-free media, supplemented with different concentrations
Figure 4. Fig 3. FBS increases sphere-forming efficiency of irradiated parotid-derived cells. Representative bright field images of salispheres grown from UT (untreated) and IR d30 (irradiated) glands in serum-free media, supplemented with different concentrations of fetal bovine serum (FBS), taken at day 4 (A) and 7 (B) in culture. Number of salispheres was quantified per 6 wells from 3 primary cell preparations per treatment group at days 4–7 in culture and expressed as average ± SEM. P values were obtained with a 2-sided unpaired t-test (n = 3), p < .01, p < .001, p < .0001 (C). Representative graphs showing the distribution of salisphere sizes among all the salispheres counted from one UT and IR d30 primary sphere preparation (D). Scale bar = 100μm.

Descrizione

Fetal bovine serum supplementation increases the sphere-forming efficiency of irradiated parotid-derived cells, as shown by representative bright-field microscopy images and quantification.

More Figures from This Paper

Sphere-forming efficiency of parotid-derived cells decreases significantly following a single 5 Gy radiation dose, quantified at day 30 post-irradiation.

Figure 2

Sphere-forming efficiency of parotid-derived cells decreases significantly following a single 5 Gy radiation dose, quantified at day 30 post-irradiation.

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Proliferation rates of salisphere cultures from untreated and irradiated parotid glands are compared under serum-free conditions, showing similar growth kinetics despite radiation damage.

Figure 3

Proliferation rates of salisphere cultures from untreated and irradiated parotid glands are compared under serum-free conditions, showing similar growth kinetics despite radiation damage.

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Proliferation assays confirm comparable growth rates between untreated and irradiated salisphere cultures supplemented with FBS, indicating that reduced sphere formation is not due to proliferation deficits.

Figure 5

Proliferation assays confirm comparable growth rates between untreated and irradiated salisphere cultures supplemented with FBS, indicating that reduced sphere formation is not due to proliferation deficits.

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Expression of salivary stem and progenitor cell markers in salisphere cultures demonstrates that both untreated and irradiated cells maintain their progenitor phenotype.

Figure 6

Expression of salivary stem and progenitor cell markers in salisphere cultures demonstrates that both untreated and irradiated cells maintain their progenitor phenotype.

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Post-radiation IGF-1 treatment significantly enhances sphere-forming efficiency of irradiated parotid cells, suggesting growth factor administration as a strategy to restore salivary function.

Figure 7

Post-radiation IGF-1 treatment significantly enhances sphere-forming efficiency of irradiated parotid cells, suggesting growth factor administration as a strategy to restore salivary function.

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Figure 4

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Source Paper

Administration of growth factors promotes salisphere formation from irradiated parotid salivary glands.

PloS one (2018)

PMID: 29590144

DOI: 10.1371/journal.pone.0193942

Cite This Figure

![Figure 4: Fetal bovine serum supplementation increases the sphere-forming efficiency of irradiated parotid-derived cells, as shown by representative bright-field microscopy images and quantification.](https://pdfs.citedhealth.com/figures/29590144/183.png)

> Source: Vicky T Nguyen et al. "Administration of growth factors promotes salisphere formation from irradiated p." *PloS one*, 2018. PMID: [29590144](https://pubmed.ncbi.nlm.nih.gov/29590144/)
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  <img src="https://pdfs.citedhealth.com/figures/29590144/183.png" alt="Fetal bovine serum supplementation increases the sphere-forming efficiency of irradiated parotid-derived cells, as shown by representative bright-field microscopy images and quantification." />
  <figcaption>Figure 4. Fetal bovine serum supplementation increases the sphere-forming efficiency of irradiated parotid-derived cells, as shown by representative bright-field microscopy images and quantification.<br>  Source: Vicky T Nguyen et al. "Administration of growth factors promotes salisphere formation from irradiated p." <em>PloS one</em>, 2018. PMID: <a href="https://pubmed.ncbi.nlm.nih.gov/29590144/">29590144</a></figcaption>
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Magnesio glicinato per Sindrome delle gambe senza riposo

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