**Figure 3.** Fig 2. Similar proliferation rates are observed in untreated and irradiated salispheres cultured in serum-free conditions. Untreated and irradiated parotid-derived salispheres were fixed after 7 and 14 days in culture and stained for Ki-67 (green). Representative confocal immunofluorescence images are shown. Ki-67 was expressed in salisphere-derived cells at both early (day 7) and later (day 14) time points in culture (A-B). Percentage of Ki-67+ proliferating cells was quantified from 20 salispheres, of different sizes (<50μm, 50–150μm), at day 7 and 14 for both treatment groups and expressed as percent of proliferation by individual salisphere (red bar mean percent of proliferation by group). At day 7 in culture, medium-sized salispheres (50–150μm) were rarely detected, thus this analysis was not determined (n.d.). Significant differences (p< 0.05) were determined using a one-way ANOVA with post-hoc Tukey multiple-comparison tests on the log transformed data of Fig 2C. Groups with the same letter are not significant different from each other (C). Scale bar 50μm.

Descrizione

Proliferation rates of salisphere cultures from untreated and irradiated parotid glands are compared under serum-free conditions, showing similar growth kinetics despite radiation damage.

More Figures from This Paper

Figure 2

Sphere-forming efficiency of parotid-derived cells decreases significantly following a single 5 Gy radiation dose, quantified at day 30 post-irradiation.

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Figure 4

Fetal bovine serum supplementation increases the sphere-forming efficiency of irradiated parotid-derived cells, as shown by representative bright-field microscopy images and quantification.

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Figure 5

Proliferation assays confirm comparable growth rates between untreated and irradiated salisphere cultures supplemented with FBS, indicating that reduced sphere formation is not due to proliferation deficits.

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Figure 6

Expression of salivary stem and progenitor cell markers in salisphere cultures demonstrates that both untreated and irradiated cells maintain their progenitor phenotype.

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Figure 7

Post-radiation IGF-1 treatment significantly enhances sphere-forming efficiency of irradiated parotid cells, suggesting growth factor administration as a strategy to restore salivary function.

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Figure 3

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Source Paper

Administration of growth factors promotes salisphere formation from irradiated parotid salivary glands.

PloS one (2018)

PMID: 29590144

DOI: 10.1371/journal.pone.0193942

Cite This Figure

![Figure 3: Proliferation rates of salisphere cultures from untreated and irradiated parotid glands are compared under serum-free conditions, showing similar growth kinetics despite radiation damage.](https://pdfs.citedhealth.com/figures/29590144/172.png)

> Source: Vicky T Nguyen et al. "Administration of growth factors promotes salisphere formation from irradiated p." *PloS one*, 2018. PMID: [29590144](https://pubmed.ncbi.nlm.nih.gov/29590144/)

<figure>
  <img src="https://pdfs.citedhealth.com/figures/29590144/172.png" alt="Proliferation rates of salisphere cultures from untreated and irradiated parotid glands are compared under serum-free conditions, showing similar growth kinetics despite radiation damage." />
  <figcaption>Figure 3. Proliferation rates of salisphere cultures from untreated and irradiated parotid glands are compared under serum-free conditions, showing similar growth kinetics despite radiation damage.<br>  Source: Vicky T Nguyen et al. "Administration of growth factors promotes salisphere formation from irradiated p." <em>PloS one</em>, 2018. PMID: <a href="https://pubmed.ncbi.nlm.nih.gov/29590144/">29590144</a></figcaption>
</figure>

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